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Image Search Results
Journal: RSC Advances
Article Title: Design and synthesis of purine nucleoside analogues for the formation of stable anti-parallel-type triplex DNA with duplex DNA bearing the 5m CG base pair
doi: 10.1039/d1ra02831f
Figure Lengend Snippet: Synthesis of amidite compounds of new nucleoside analogues and TFOs. Reagents and conditions: (a) DMTrCl, pyridine, 92%, (b) (1) ethylenediamine, EtOH, 60 °C, (2) FmocCl, Et 3 N, CH 2 Cl 2 , 57% in 2 steps, (c) TBSCl, imidazole, CH 3 CN, 86%, (d) (1) PdAc 2 , xantphos, toluene, 3-aminophenyl benzoate, Cs 2 CO 3 , (2) Et 3 N–3HF, Et 3 N, THF, 80% in two steps, (e) (1) PdAc 2 , xantphos, toluene, 2-aminophenyl benzoate, NaO t Bu, (2) Bz 2 O, DMAP, (3) Et 3 N–3HF, Et 3 N, THF, 35% in three steps, (f) 2-cyanoethyl- N , N -diisopropylchlorophosphoramidite, DIPEA, CH 2 CH 2 , 0 °C, 49, 63, or 66% for 10, 11, or 12, respectively, (g) DNA automated synthesizer, followed by 28% ammonia solution at 55 °C for 12 h, HPLC purification, and then 5% acetic acid.
Article Snippet: Cleavage from the resin was accomplished by an overnight treatment with 28% ammonium hydroxide at 55 °C for 5 h, followed by
Techniques: Analogues, Purification